Stem cells, mainly mesenchymal stem cells and induced pluripotent stem cells (iPSC), were used to treat common myocardial ischemia, lower limb ischemia, renal ischemia, and other diseases. Preparation of stem cell transplantation vector materials, mainly active hydrogels, to regulate the proliferation, differentiation, and secretion of stem cells and improve the survival rate of transplanted stem cells; To develop novel molecular probes for tracer study of transplanted stem cells and reveal the behavior, function, and fate of transplanted stem cells in vivo; Using materials and engineering methods to process stem cells and regulate the function of stem cells, including exosome secretion and exosome function regulation; Functionalized culture of stem cells can improve the proliferation ability and anti-apoptosis ability of stem cells.